Automatic pipeting attachment for the Van Slyke manometric apparatus for CO2 determination.

N AUTOMATIC PIPETING DEVICE for measuring small samples of serum or other biologic fluids has been described (1). This type of pipet can be used to measure 0.1 to 0.2 ml. volumes of different samples accurately. It is self-cleaning and can be used repeatedly from sample to sample. Because of these desirable features it was applied to the manometric Van Slyke apparatus (2) for the measurement of CO2. Using this measuring device and the calibrations on the extraction chamber, the CO2 in 0.1 to 0.2 ml. of serum can be measured to ± 0.3 mMol per liter without the use of pipets. Furthermore, this modification increases the efficiency of the procedure.